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9002-12-4

Basic Information
CAS No.: 9002-12-4
Name: URICASE
Molecular Structure:
Molecular Structure of 9002-12-4 (URICASE)
Formula: C18H26N5O14P
Molecular Weight: 567.397901
Synonyms: E.C.1.7.3.3;Uratoxidase;Uric acidoxidase;Uricase;Uricozyme;
EINECS: 232-655-5
Solubility: hardly soluble in water, slightly soluble in buffer alkaline solution
Appearance: lyophilized powder
Safety: 24/25
PSA: 0.00000
LogP: 0.00000
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Specification

The Urate oxidase, with the CAS registry number 9002-12-4, is also known as Uricozyme. It belongs to the product categories of 1.7.x.x Other nitrogenous donors; 1.x.x.x Oxidoreductases; General Metabolic Enzymes; General Metabolic Enzymes I-ZEnzyme Class Index; Metabolomics. Its EINECS number is 232-655-5. Its EC number is 1.7.3.3. Its classification codes are: (1)Drug / Therapeutic Agent; (2)Natural Product; (3)TSCA Flag XU [Exempt from reporting under the Inventory Update Rule]. It should be sealed and stored in a ventilated and dry place. Its storage temperature is -20 °C. Moreover, it should be protected from light. Urate oxidase is mainly localised in the liver, where it forms a large electron-dense paracrystalline core in many peroxisomes. The enzyme exists as a tetramer of identical subunits, each containing a possible type 2 copper-binding site. Urate oxidase is a homotetrameric enzyme containing four identical active sites situated at the interfaces between its four subunits. It can be found in an extensive variety of organisms, from bacteria to mammals, and plays different metabolic roles, depending on its host organism, it is absent in humans and many other primates.

Preparation: with yeast as raw materials, extract with sodium phosphate, then grade with ammonium sulfate. The product is obtained through dialysis, column chromatography, ammonium sulfate recrystallization.

Uses of Urate oxidase: the enzyme Urate oxidase (UO), or uricase or factor-independent urate hydroxylase, catalyzes the oxidation of uric acid to 5-hydroxyisourate.

Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2

The toxicity data is as follows:

Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
mouse LD oral > 2500mg/kg (2500mg/kg)   Kiso to Rinsho. Clinical Report. Vol. 6, Pg. 1914, 1972.
rat LD oral > 1gm/kg (1000mg/kg)   Kiso to Rinsho. Clinical Report. Vol. 6, Pg. 1914, 1972.