Edeine binds to 70-S ribosomes in the presence of 10 mM MgCl2. At a concentration of Mg2+ of 1 mM the binding is 30 times greater than at 10 mM. Edeine does not affect the formation of polysomes, although some binding of edeine to polysomes is observed. Edeine causes association of 30-S and 50-S...detailed
Enzymes obtained from cells disrupted by lysozyme treatment were resolved into three complementary enzyme fractions by DEAE-cellulose-Sephadex G-200 chromatography. These enzymes of molecular weight 210 000 (A), 180 000 (B) and 100 000 (C) were responsible for the activation of the constituent a...detailed
Publisher SummaryThis chapter discusses the assay and purification procedure of edeine synthetases. In assay method, binding of the amino acyl to the enzyme complex can be measured only if radioactive edeine constituent amino acids are available. Formation of edeine A and B is measured by incorp...detailed
Edeine inhibits the synthesis of bacterial DNA as shown by the incorporation studies of [3H]thymidine into DNA of Escherichia coli 15 T−. It does not induce a release of [3H]thymidine from prelabeled DNA. Edeine does not bind to the DNA. DNA isolated from edeine treated Bacillus subtilis shows t...detailed
1.1. Exogenous edeine inhibits the synthesis of DNA and protein, but not that of RNA, in extracts of edeine-producing Bacillus brevis Vm 4 cells. This is analogous to the effect of edeine on extracts obtained from edeine-sensitive cells.2.2. Producer cells, in contrast to sensitive ones, are not...detailed
The crystal structures of the universal translation-initiation inhibitors edeine and pactamycin bound to ribosomal 30S subunit have revealed that edeine induces base pairing of G693:C795, residues that constitute the pactamycin binding site. Here, we show that base pair formation by addition of ...detailed
SummaryEdeines are atypical cationic peptides produced by Brevibacillus brevis Vm4 with broad-spectrum antimicrobial activity. These linear nonribosomal peptides bind to the 30S ribosomal subunit and block t-RNA binding to the P-site. To identify the mechanism of high-level self-resistance in th...detailed