116919-18-7 Usage
Uses
Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides.
General Description
Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups.
Check Digit Verification of cas no
The CAS Registry Mumber 116919-18-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,6,9,1 and 9 respectively; the second part has 2 digits, 1 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 116919-18:
(8*1)+(7*1)+(6*6)+(5*9)+(4*1)+(3*9)+(2*1)+(1*8)=137
137 % 10 = 7
So 116919-18-7 is a valid CAS Registry Number.
InChI:InChI=1/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1
116919-18-7Relevant articles and documents
Method for preparing biotin maleimide
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Paragraph 0025; 0040; 0045; 0046; 0051, (2019/04/06)
The invention discloses a method for preparing biotin maleimide. The method comprises the following steps of: 1, reacting 6-maleimidohexanoic acid compound (1) with N-hydroxysuccinimide under the action of a condensing agent to obtain a 6-maleimidohexanoic acid N-hydroxysuccinimide ester compound (2); 2, treating the 6-maleimidohexanoic acid N-hydroxysuccinimide ester compound (2) by using hydrazine hydrate to obtain a 6- maleimidocaproyl hydrazide compound (3); 3, reacting a biotin compound (4) with an acyl halide reagent to obtain a biotinyl acyl halide compound (5); and 4, reacting the 6-maleimidocaproyl hydrazide compound (3) with the biotinyl acyl halide compound (5) to obtain a biotin maleimide compound (6). The method for preparing the biotin maleimide, disclosed by the invention, has the beneficial effects of being suitable for scale-up production, easily available in raw materials, simple in preparation process and friendly to environment.
