14197-60-5Relevant articles and documents
The preparation of ginsenoside Rg5, its antitumor activity against breast cancer cells and its targeting of PI3K
Liu, Yannan,Fan, Daidi
, (2020/02/11)
Ginsenosides have been reported to possess various pharmacological effects, including anticancer effects. Nevertheless, there are few reports about the antitumor activity and mechanisms of ginsenoside Rg5 against breast cancer cells. In the present study, the major ginsenoside Rb1 was transformed into the rare ginsenoside Rg5 through enzymatic bioconversion and successive acid-assisted high temperature and pressure processing. Ginsenosides Rb1, Rg3, and Rg5 were investigated for their antitumor effects against five human cancer cell lines via the MTT assay. Among them, Rg5 exhibited the greatest cytotoxicity against breast cancer. Moreover, Rg5 remarkably suppressed breast cancer cell proliferation through mitochondria-mediated apoptosis and autophagic cell death. LC3B-GFP/Lysotracker and mRFP-EGFP-LC3B were utilized to show that Rg5 induced autophagosome-lysosome fusion. Western blot assays further illustrated that Rg5 decreased the phosphorylation levels of PI3K, Akt, mTOR, and Bad and suppressed the PI3K/Akt signaling pathway in breast cancer. Moreover, Rg5-induced apoptosis and autophagy could be dramatically strengthened by the PI3K/Akt inhibitor LY294002. Finally, a molecular docking study demonstrated that Rg5 could bind to the active pocket of PI3K. Collectively, our results revealed that Rg5 could be a potential therapeutic agent for breast cancer treatment.
Manufacturing method for mass-production of 20(S)-ginsenoside Rg3
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Paragraph 0029; 0031-0043, (2018/08/30)
The present invention relates to a method for selectively mass-producing 20(S)-ginsenoside Rg3 from ginsenoside Rd. The 20(S)-ginsenoside Rg3 rarely exists in white ginseng or red ginseng, and exists as an S-type isomer or an R-type isomer even though exiting in a small amount. Thus, an amount thereof refined as a single material is very low, and thus a use thereof is not easy. However, according to the present invention, 20(S)-ginsenoside Rg3 is mass-produced by using only ginsenoside Rd which can be easily separated from leaves of ginsengs, and thus addition of ginsenoside which may be generated from Rc, Re, Rf, and the like can be relatively prevented compared to when extracts of ginsengs, red ginsengs, or the like are used. A relative production amount of 20(R)-ginsenoside Rg3 is low. Thus, purification is easy, and mass-production is easy.(AA) Before steaming(BB) Example 1-1 (dry steaming, 120 anddeg;C, 2 hours, 0.13MPa)(CC) Example 1-2 (dry steaming, 120 anddeg;C, 4 hours, 0.13MPa)(DD) Example 1-3 (dry steaming, 120 anddeg;C, 6hours, 0.13MPa)COPYRIGHT KIPO 2018
Biotransformation of ginsenoside Rd into 20(S)-Rg3 by bacterium flavobacterium sp. BGS36
Ten,Chae,Yoo
, p. 181 - 183 (2014/06/09)
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