1596-56-1 Usage
Chemical Properties
solid
Uses
Naphthol AS-MX phosphate has been used as:a substrate for alkaline phosphatase in human osteoblastsmouse embryonic stem cell (mESC) coronal plane sections for fluorescence staining and analysis
General Description
Naphthol AS-MX phosphate is soluble in dioxane and is a substrate for phosphatase.
Check Digit Verification of cas no
The CAS Registry Mumber 1596-56-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,5,9 and 6 respectively; the second part has 2 digits, 5 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 1596-56:
(6*1)+(5*5)+(4*9)+(3*6)+(2*5)+(1*6)=101
101 % 10 = 1
So 1596-56-1 is a valid CAS Registry Number.
InChI:InChI=1/C19H18NO5P/c1-12-7-8-17(13(2)9-12)20-19(21)16-10-14-5-3-4-6-15(14)11-18(16)25-26(22,23)24/h3-11H,1-2H3,(H,20,21)(H2,22,23,24)
1596-56-1Relevant articles and documents
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
