173471-71-1Relevant articles and documents
Reaction-based two-photon probes for mercury ions: Fluorescence imaging with dual optical windows
Rao, Alla Sreenivasa,Kim, Dokyoung,Wang, Taejun,Kim, Ki Hean,Hwang, Sekyu,Ahn, Kyo Han
, p. 2598 - 2601 (2012)
For fluorescent imaging of mercury ions in living species, two-photon probes with dual optical windows are in high demand but remain unexplored. Several dithioacetals were evaluated, and a probe was found, which, upon reaction with mercury species, yielded a two-photon dye; this conversion accompanies ratiometric emission changes with a 97-nm shift, enabling fluorescent imaging of both the probe and mercury ions in cells by one- and two-photon microscopy for the first time.
Readily Accessible and Predictable Naphthalene-Based Two-Photon Fluorophore with Full Visible-Color Coverage
Koo, Ja Young,Heo, Cheol Ho,Shin, Young-Hee,Kim, Dahahm,Lim, Chang Su,Cho, Bong Rae,Kim, Hwan Myung,Park, Seung Bum
, p. 14166 - 14170 (2016)
Herein we report 22 acedan-derived, two-photon fluorophores with synthetic feasibility and full coverage of visible wavelength emission. The emission wavelengths were predicted by computational analysis, which enabled us to visualize multicolor images by two-photon excitation with single wavelength, and to design a turn-on, two-photon fluorescence sensor for endogenous H2O2in Raw 264.7 macrophage and rat brain hippocampus ex vivo.
Naphthalene derivatives of a conformationally locked GFP chromophore with large stokes shifts
Baleeva, Nadezhda S.,Khavroshechkina, Anastasia V.,Zaitseva, Elvira R.,Myasnyanko, Ivan N.,Zagudaylova, Marina B.,Baranov, Mikhail S.
, (2019)
The active development of fluorescence microscopy requires an increase in the variety of the dyes and their characteristics. Compounds with a large Stokes shift, i.e., a large difference between the positions of the absorption and emission maxima, have at
ICT-based fluorescent ratiometric probe for monitoring mitochondrial peroxynitrite in living cells
Du, Yuting,Wang, Hongliang,Zhang, Ting,Wei, Wen,Guo, Minmin
supporting information, p. 12915 - 12921 (2021/08/03)
Peroxynitrite (ONOO?) is a reactive oxygen species (ROS) that causes serious damage to living cells and is the cause of a series of human diseases. It is reported that mitochondria are the major site of ONOO?production. Therefore, accurate and rapid detection of intracellular ONOO?is important in pathological processes. Herein, a mitochondria-targeted ratiometric fluorescent probe (DMANI) has been rationally designed based on the coumarin-hemicyanine hybrid framework by regulating the intramolecular charge transfer (ICT) effect. TheDMANIcan capture ONOO?at low concentrationsviadirect addition with the CC bond to give an obvious fluorescent signal change from red to blue based on the ICT process. The possible mechanism for this electrophilic addition process was confirmed using electrospray ionization mass spectrometry (ESI-MS) and1H-NMR spectra.DMANIexhibited a good selectivity, a large Stokes shift (248 nm), a low detection limit (40 nM), and a rapid response (within 20 s) to ONOO?. More importantly,DMANIwas successfully used for ratiometric imaging and monitoring of the fluctuations of mitochondrial ONOO?in living cells.
A Fluorescent Kinase Inhibitor that Exhibits Diagnostic Changes in Emission upon Binding
Fleming, Cassandra L.,Sandoz, Patrick A.,Inghardt, Tord,?nfelt, Bj?rn,Gr?tli, Morten,Andréasson, Joakim
, p. 15000 - 15004 (2019/09/17)
The development of a fluorescent LCK inhibitor that exhibits favourable solvatochromic properties upon binding the kinase is described. Fluorescent properties were realised through the inclusion of a prodan-derived fluorophore into the pharmacophore of an ATP-competitive kinase inhibitor. Fluorescence titration experiments demonstrate the solvatochromic properties of the inhibitor, in which dramatic increase in emission intensity and hypsochromic shift in emission maxima are clearly observed upon binding LCK. Microscopy experiments in cellular contexts together with flow cytometry show that the fluorescence intensity of the inhibitor correlates with the LCK concentration. Furthermore, multiphoton microscopy experiments demonstrate both the rapid cellular uptake of the inhibitor and that the two-photon cross section of the inhibitor is amenable for excitation at 700 nm.
