10019-03-1 Usage
General Description
Naphthol AS-BS phosphate is a chemical compound used as a substrate for detecting and quantifying alkaline phosphatase activity in biochemical assays. It is a colorless to pale yellow solid with a phenolic odor that is soluble in water. When combined with alkaline phosphatase, it undergoes a hydrolysis reaction, resulting in the formation of a colored product that can be measured spectrophotometrically. Naphthol AS-BS phosphate is commonly used in enzyme-linked immunosorbent assays (ELISAs) and other detection methods for studying enzyme kinetics, diagnosing diseases, and conducting biomedical research. However, it should be handled and disposed of with care, as it can cause irritation to the eyes, skin, and respiratory tract, and may be harmful if ingested or inhaled.
Check Digit Verification of cas no
The CAS Registry Mumber 10019-03-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,0,1 and 9 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 10019-03:
(7*1)+(6*0)+(5*0)+(4*1)+(3*9)+(2*0)+(1*3)=41
41 % 10 = 1
So 10019-03-1 is a valid CAS Registry Number.
InChI:InChI=1/C17H13N2O7P/c20-17(18-13-6-3-7-14(10-13)19(21)22)15-8-11-4-1-2-5-12(11)9-16(15)26-27(23,24)25/h1-10H,(H,18,20)(H2,23,24,25)
10019-03-1Relevant articles and documents
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
