1004620-06-7Relevant academic research and scientific papers
The aqueous N-phosphorylation and N-thiophosphorylation of aminonucleosides
Conway, Louis P.,Delley, Richard J.,Neville, Jonathan,Freeman, Gemma R.,Maple, Hannah J.,Chan, Vincent,Hall, Alexander J.,Hodgson, David R. W.
, p. 38663 - 38671 (2014)
We demonstrate N-phosphorylation and N-thiophosphorylation of unprotected aminonucleosides in aqueous media. N-Phosphorylations using phosphoric chloride and N-thiophosphorylations using thiophosphoryl chloride were explored as functions of pH using 5′-amino-5′-deoxyguanosine as substrate. These reagents were compared to phosphodichloridate and thiophosphodichloridate ions, and the methodology was applied to other aminonucleosides. S-Alkylations of the nucleoside N-thiophosphoramidates were investigated as functions of pH and alkylating agent. the Partner Organisations 2014.
Preparation and purification of 5′-amino-5′-deoxyguanosine- 5′-N-phosphoramidate and its initiation properties with T7 RNA polymerase
Williamson, David,Hodgson, David R. W.
, p. 1056 - 1062 (2008)
5′-Amino-5′-deoxyguanosine-5′-N-phosphoramidate (GNHP) was synthesised in four steps from guanosine. Its identity was confirmed using spectroscopic and kinetic studies. A chromatographic method for the purification of this unstable compound was developed. GNHP was found to initiate T7 RNAP promoted transcriptions to afford 5′-O3P-NH-RNA, which hydrolyses readily to yield 5′-H2N-RNA that can be conjugated to activated esters.
