1007364-35-3Relevant academic research and scientific papers
Hapten synthesis and monoclonal antibody-based immunoassay development for the detection of the fungicide kresoxim-methyl
Mercader, Josep V.,Suarez-Pantaleon, Celia,Agullo, Consuelo,Abad-Somovilla, Antonio,Abad-Fuentes, Antonio
experimental part, p. 1545 - 1552 (2009/07/18)
Strobilurin fungicides have been increasingly used for fungus pest control since they were introduced in 1996. For pesticide residue detection, immunoassays constitute nowadays a valuable approach. This paper describes the synthesis of functionalized haptens of kresoxim-methyl, the production of monoclonal antibodies, and the development of enzyme-linked immunosorbent assays. On the one hand, a two-step conjugate-coated immunoassay was optimized using extended or short incubation times, with limits of detection of 0.4 ng/mL for the extended assay and 0.3 ng/mL for the rapid assay. On the other hand, an immunoassay was optimized following a procedure consisting of just one incubation step. This one-step assay had a limit of detection of 0.4 ng/mL. All of these assays showed a similar performance, with sensitivities well below common maximum residue limits for this pesticide (50 μg/kg) and lower than the detection limits of the usual chromatographic detection methods.
