10121-91-2 Usage
Description
Monodansylcadaverine (MDC) is a fluorescent marker for autophagic vacuoles. It is an autofluorescent substance incorporated into multilamellar bodies by both an ion trapping mechanism and interaction with membrane lipids, exhibiting a Stokes shift and increased relative fluorescence in hydrophobic environments. MDC selectively accumulates in PaTu 8902 subcellular fractions containing the lysosomal enzymes acid phosphatase and cathepsin D, but not those containing the rough and smooth endoplasmic reticulum markers TRAM and cytochrome P450, respectively.
Uses
Different sources of media describe the Uses of 10121-91-2 differently. You can refer to the following data:
1. Fluorescent substrate for the assay of transamidating enzymes.
2. Lysosomotropic fluorescent Probe
3. Dansylcadaverine is a fluorescent amine probe, which is believed to directly inhibit vesicular transport. Studies indicate that Dansylcadaverine can act as a marker used to study autophagy in the cytoplasm and vacuoles of plants and mammalian cells.
References
1) Munafo and Colombo (2001),?A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation; Int. J. Cell Sci.,?114?3619
2) Jiang?et al. (2012),?Targeting androgen receptor leads to suppression of prostate cancer via induction of autophagy; J. Urol.,?188?1361
3) Haigler?et al.?(1980),?Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells; J. Biol. Chem., 255 1239
4) Murthy?et al. (1994),?Residue Gln-30 of human erythrocyte anion transporter is a prime site for reaction with intrinsic transglutaminase; J. Biol. Chem.,?269?22907
Check Digit Verification of cas no
The CAS Registry Mumber 10121-91-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,1,2 and 1 respectively; the second part has 2 digits, 9 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 10121-91:
(7*1)+(6*0)+(5*1)+(4*2)+(3*1)+(2*9)+(1*1)=42
42 % 10 = 2
So 10121-91-2 is a valid CAS Registry Number.
InChI:InChI=1/C18H27N3O2S/c1-21(2)17-11-7-10-16-15(17)9-8-12-18(16)24(22,23)20-14-6-4-3-5-13-19/h7-12,20H,3-6,13-14,19H2,1-2H3
10121-91-2Relevant articles and documents
Expanding the rubterolone family: Intrinsic reactivity and directed diversification of PKS-derived pyrans
Guo, Huijuan,Benndorf, René,K?nig, Stefanie,Leichnitz, Daniel,Weigel, Christiane,Peschel, Gundela,Berthel, Patrick,Kaiser, Marcel,Steinbeck, Christoph,Werz, Oliver,Poulsen, Michael,Beemelmanns, Christine
, p. 11319 - 11324 (2018)
We characterized two key biosynthetic intermediates of the intriguing rubterolone family (tropolone alka-loids) that contain a highly reactive pyran moiety (in equilibrium with the hydrolyzed 1,5-dione form) and undergo spontaneous pyridine formation in the presence of primary amines. We exploited the intrinsic reactivity of the pyran moiety and isolated several new rubterolone derivatives, two of which contain a unique thiazolidine moiety. Three rubterolone derivatives were chemically modified with fluorescence and biotin tags using peptide coupling and click reaction. Overall, eight derivatives were fully characterized by HRMS/MS and 1D and 2D NMR spectroscopy and their antimicrobial, cytotoxic, anti-inflammatory and antiparasitic activities evaluated.
