101869-81-2Relevant articles and documents
Regulated expression of the Escherichia coli lepB gene as a tool for cellular testing of antimicrobial compounds that inhibit signal peptidase I in vitro
Barbosa, Maria D. F. S.,Lin, Siqi,Markwalder, Jay A.,Mills, Jonathan A.,DeVito, Joseph A.,Teleha, Christopher A.,Garlapati, Vasudha,Liu, Charles,Thompson, Andy,Trainor, George L.,Kurilla, Michael G.,Pompliano, David L.
, p. 3549 - 3554 (2002)
Escherichia coli under-expressing lepB was utilized to test cellular inhibition of signal peptidase I (SPase). For the construction of a lepB regulatable strain, the E. coli lepB gene was cloned into pBAD, with expression dependent on L-arabinose. The chr