102185-33-1 Usage
Uses
Used in Molecular Biology and Histology:
5-Bromo-4-chloro-3-indolyl phosphate disodium salt is used as a colorimetric detection agent for alkaline phosphatase-labeled molecules. The BCIP/NBT substrate system is a versatile tool that can be applied in various techniques such as Northern, Southern, and Western blotting, in situ hybridization, and immunohistochemistry. The colorimetric detection allows for the visualization and quantification of target molecules, making it a valuable tool in research and diagnostic applications.
Used in Diagnostic Applications:
In the field of diagnostics, 5-Bromo-4-chloro-3-indolyl phosphate disodium salt is used as a component in enzyme-linked immunosorbent assays (ELISA) and other immunoassay techniques. The colorimetric change upon interaction with alkaline phosphatase enables the detection and quantification of specific antibodies or antigens, aiding in the diagnosis of various diseases and conditions.
Used in Research and Development:
5-Bromo-4-chloro-3-indolyl phosphate disodium salt is also utilized in research and development for the study of alkaline phosphatase activity and its role in various biological processes. The compound can be used to investigate the expression and regulation of alkaline phosphatase in different cell types, tissues, and organisms, contributing to a better understanding of its function and potential therapeutic applications.
Check Digit Verification of cas no
The CAS Registry Mumber 102185-33-1 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,2,1,8 and 5 respectively; the second part has 2 digits, 3 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 102185-33:
(8*1)+(7*0)+(6*2)+(5*1)+(4*8)+(3*5)+(2*3)+(1*3)=81
81 % 10 = 1
So 102185-33-1 is a valid CAS Registry Number.
InChI:InChI=1/C8H6BrClNO4P/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14/h1-3,11H,(H2,12,13,14)/p-2
