10232-92-5Relevant articles and documents
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Byalaya,E.I. et al.
, (1976)
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Synthetic method of D,L-pantolactone
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Paragraph 0056; 0059-0060; 0061; 0064-0065; 0066; 0069-0070, (2019/12/09)
The invention belongs to the field of chemical technology, and more specifically relates to a synthetic method of D,L-pantolactone. The synthetic method of D,L-pantolactone comprises following steps:paraformaldehyde and isobutyraldehyde are subjected to aldol condensation reaction under the effect of an alcohol solvent and an alkaline catalyst, and an obtained reaction solution is directly used in a next step treatment without separation; hydrocyanic acid is added, reaction is carried out to generate 2, 4-dihydroxyl-3, 3-dimethylbutyronitrile; 2, 4-dihydroxyl-3, 3-dimethylbutyronitrile is subjected to esterification reaction under acidic conditions to obtain 2, 4-dihydroxyl-3, 3-dimethyl butyrate; an alkali is added for neutralizing of excess acid, and solid liquid separation is carried out to obtain a side product ammonium salt; liquid evaporation is carried out for alcohol recycling, and rectification is carried out to obtain D,L-pantolactone product. The synthetic method of D,L-pantolactone is capable of preparing a target product high in content and yield, reducing acid and alkali using amount, avoiding using of organic solvents in production extraction, reducing generated waste water greatly, saving energy, protecting the environment, and reducing cost.
Nitrile hydratase and a method for producing amides
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, (2008/06/13)
An objective of the present invention is to provide a nitrile hydratase capable of producing 2-hydroxy-4-methylthiobutyroamide. The present invention provides a novel nitrile hydratase producing α-hydroxyamide using, α-hydroxnitrile as the substrate, and the encoding DNA thereof. The enzyme can be obtained from Rhodococcus sp. Further, the enzymatic activity of the enzyme can he maintained stably during the reaction. The present invention provides a method for producing amide compounds, the method comprising the step of reacting this enzyme to nitrile compounds. According to the present invention, from hydroxy nitrile compounds, corresponding amide compounds can be produced biochemically without reducing the enzyme activity of nitrile hydratase.