1026020-97-2Relevant academic research and scientific papers
Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl tranferase. Application in electrochemical DNA hybridization and protein-DNA binding assays
Horakova, Petra,MacIkova-Cahova, Hana,Pivokova, Hana,Paek, Jan,Havran, Ludk,Hocek, Michal,Fojta, Miroslav
experimental part, p. 1366 - 1371 (2011/04/16)
A simple approach to DNA tail-labelling using terminal deoxynucleotidyl transferase and modified deoxynucleoside triphosphates is presented. Amino- and nitrophenyl-modified dNTPs were found to be good substrates for this enzyme giving 3′-end stretches of
Aminophenyl- and nitrophenyl-labeled nucleoside triphosphates: Synthesis, enzymatic incorporation, and electrochemical detection
Cahova, Hana,Havran, Ludek,Brazdilova, Petra,Pivonkova, Hana,Pohl, Radek,Fojta, Miroslav,Hocek, Michal
supporting information; experimental part, p. 2059 - 2062 (2009/02/06)
(Chemical Equation Presented) Primer extension is used to incorporate labeled nucleoside triphosphates into oligonucleotides (ONs). Aminophenyl and nitrophenyl modifications serve as electrochemical labels that are detectable by either oxidation or reduct
