1027269-81-3Relevant academic research and scientific papers
Homogeneous fluorescence assays for RNA diagnosis by pyrene-conjugated 2′-O-methyloligoribonucleotides
Sakamoto, Takashi,Kobori, Akio,Shigezawa, Masako,Amitani, Yasutaka,Higuchi, Maiko,Murakami, Akira
, p. 1659 - 1664 (2008/09/20)
We developed a bispyrene-conjugated 2′-O-methyloligoribonucleotide as an RNA-specific RNA-probe. The probe hybridized with the complementary RNA, greatly enhancing fluorescence and discriminating RNA from DNA. The assay was carried out in homogeneous aqueous media without removing the unbound probe from the detection solution. This homogeneous fluorescence assay also discriminated mismatch sequences in the target RNA. These pyrene probes could possess high potential to detect RNA in biological specimens simply. Copyright Taylor & Francis Group, LLC.
Pyrene aromatic arrays on RNA duplexes as helical templates
Nakamura, Mitsunobu,Shimomura, Yukinori,Ohtoshi, Yukinori,Sasa, Kazuhiro,Hayashi, Haruhisa,Nakano, Hidehiko,Yamana, Kazushige
, p. 1945 - 1951 (2008/02/10)
RNA oligomers having multiple (2 to 4) pyrenylmethyl substituents at the 2′-O-sugar residues were synthesized. UV-melting studies showed that the pyrene-modified RNAs could form duplexes with complementary RNA sequences without loss of thermal stability.
