1037807-52-5Relevant academic research and scientific papers
Flexibility of C3h-Symmetrical Linkers in Tris-oligonucleotide-Based Tetrahedral Scaffolds
Panagiotidis, Christos,Kath-Schorr, Stephanie,Von Kiedrowski, Günter
, p. 254 - 259 (2016)
Flexibility of tris-oligonucleotides is determined by the length of their connecting hydrocarbon chains. Tris-oligonucleotides are branched DNA building blocks with three oligonucleotide arms attached to a C3h-symmetrical linker core at these chains. Four tris-oligonucleotides hybridise into a tetrahedral nanocage by sequence-determined self-assembly. The influence of methylene, ethylene and propylene chains was studied by synthesising sets of tris-oligonucleotides and analysing the relative stability of the hybridisation products against digestion by mung bean nuclease by using gel electrophoresis. Linkers with ethylene chains showed sufficient flexibility, whereas methylene-chain linkers were too rigid. Tris-oligonucleotides based on the latter still formed tetrahedral scaffolds in intermixing experiments with linkers of higher flexibility. Thus, a new generation of versatile isocyanurate-based linkers was established.
Self-assembly of a DNA dodecahedron from 20 trisoligonucleotides with C3h linkers
Zimmermann, Jan,Cebulla, Martin P. J.,Moenninghoff, Sven,Von Kiedrowski, Guenter
experimental part, p. 3626 - 3630 (2009/02/08)
(Figure Presented). Can 20 trisoligonucleotides with 20 x 3 individual sequences be programmed to self-assemble into a DNA dodecahedron? The answer is yes if one starts from a new generation of trisoligonucleotides based on C 3h-symmetric linkers with proper flexibility. The resulting dodecahedron has C1 symmetry and may facilitate the construction of multimodular scaffolds in the future.
