1038399-75-5Relevant academic research and scientific papers
Fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay based on the aggregation-induced emission of silole
Wang, Ming,Zhang, Deqing,Zhang, Guanxin,Tang, Yanli,Wang, Shu,Zhu, Daoben
, p. 6443 - 6448 (2008)
By making use of the aggregation-induced emission feature of silole, compound 1 with an ammonium group is designed and synthesized with a view to developing a new optical probe for fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay. The fluorescence of 1 increases largely upon mixing with DNA, in particular for long DNA, indicating that 1 can be used for fluorescence turn-on detection of DNA. More interestingly, 1 can be employed to follow the DNA cleavage process by nuclease. Therefore, a label-free fluorescence nuclease assay method is successfully established with 1. Furthermore, this label-free fluorescence assay can also be used for inhibitor screening of nucleases. Given its simplicity, easy operation, sensitivity and cost-effectiveness, this method can be extended to other nuclease assays and high-throughput screening of nuclease inhibitors.
The convenient fluorescence turn-on detection of heparin with a silole derivative featuring an ammonium group
Wang, Ming,Zhang, Deqing,Zhang, Guanxin,Zhu, Daoben
body text, p. 4469 - 4471 (2009/05/06)
A new silole derivative with an ammonium group was designed and studied with a view to developing a convenient fluorescence turn-on assay for heparin by taking advantage of the aggregation-induced enhanced emission (AIE) feature of silole (silacyclopentadiene) molecules. The Royal Society of Chemistry.
