1042435-72-2Relevant academic research and scientific papers
A novel linker methodology for the synthesis of tailored conjugate vaccines composed of complex carbohydrate antigens and specific TH-cell peptide epitopes
Dziadek, Sebastian,Jacques, Sandra,Bundle, David R.
supporting information; experimental part, p. 5908 - 5917 (2009/05/31)
Pathogenic organisms or oncogenically transformed cells often express complex carbohydrate structures at their cell surface, which are viable targets for active immunotherapy. We describe here a novel, immunologically neutral, linker methodology for the efficient preparation of highly defined vaccine conjugates that combine complex saccharide antigens with specific T H-cell peptide epitopes. This novel heterobifunctional approach was employed for the conjugation of a (1→-2)-β-mannan trisaccharide from the pathogenic fungus Candida albicans as well as the carbohydrate portion of tumor-associated ganglioside GM2 to a TH-cell peptide epitope derived from the murine 60 kDa self heat-shock protein (hsp60). Moreover, the linkage chemistry has proven well suited for the synthesis of more complex target structures such as a biotinylated glycopeptide, a three component vaccine containing an immunostimulatory peptide epitope from interleukin-1β (IL-1β), and for the conjugation of complex carbohydrates to carrier proteins such as bovine serum albumin.
