The functional importance of glycolipids has emphasized the need for more sensitive methods of detection, characterization, and quantification than has often been possible using traditional thin-layer chromatographic techniques. We describe the use of ceramide glycanase and HPLC to identify and ...detailed
Interest in cellular glycosphingolipid (GSL) function has necessitated the development of a rapid and sensitive method to both analyze and characterize the full complement of structures present in various cells and tissues. An optimized method to characterize oligosaccharides released from glyco...detailed
Publisher SummaryCeramide glycanase (CGase) is an endoglycosidase that releases the intact glycan chain from various glycosphingolipids by cleaving the linkage between the ceramide and the glycan chain. This enzyme is found in the leech Macrobdella and in other annelids such as the European leec...detailed
Publisher SummaryThis chapter describes various methods for preparing ceramide glycanases (cer-glycanase) from leech (Hirudo medicinalis) and earthworm (Lumbricus terrestris). These organisms are suitable for the structural analysis of ceramide glycans. Because cer-glycanase can release the inta...detailed
An alkyl lactoside containing two different fluorescence probes as an energy donor and an energy acceptor was synthesized as a substrate for ceramide glycanase. n-Pentenyl β-lactoside was converted into its 4′,6′-O-(2-naphthylmethylidene) derivative with subsequent benzoylation of all remaini...detailed