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N-succinimidyl-3-(2-pyridyldithio)butyrate, commonly referred to as SPDP, is a heterobifunctional crosslinking reagent that plays a pivotal role in the field of bioconjugation and protein crosslinking. This reagent is characterized by the presence of a succinimidyl ester group, which facilitates covalent attachment to primary amines, and a pyridyldithio reactive group, which is adept at thiol modification. The unique structure of SPDP allows for the creation of stable bonds between proteins and other biomolecules or surfaces, which is instrumental in a variety of research and diagnostic applications.

107348-47-0

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107348-47-0 Usage

Uses

Used in Bioconjugation:
N-succinimidyl-3-(2-pyridyldithio)butyrate is used as a crosslinking agent for bioconjugation, allowing for the covalent attachment of proteins to other biomolecules. This is crucial for the development of conjugated systems that can be used in various biological assays and for the study of molecular interactions.
Used in Protein Crosslinking Experiments:
In protein crosslinking experiments, SPDP is utilized as a reagent to create stable bonds between proteins, which is essential for understanding protein-protein interactions and the structural dynamics within protein complexes.
Used in Immunoassays:
N-succinimidyl-3-(2-pyridyldithio)butyrate is used as a protein immobilization reagent in immunoassays. The ability to attach proteins covalently to assay plates or other surfaces ensures that the proteins remain stable and accessible for interaction with antibodies or other binding partners, enhancing the sensitivity and reliability of the assay.
Used in Affinity Chromatography:
In the field of affinity chromatography, SPDP serves as a key component for immobilizing target proteins onto chromatography matrices. This immobilization is vital for the purification and separation of specific biomolecules based on their affinity to the immobilized protein.
Used in Redox-Mediated Release of Biomolecules:
The presence of a cleavable disulfide bond in SPDP allows for the controlled release of linked biomolecules under reducing conditions. This feature is particularly useful in applications where reversible conjugation is desired, such as in the development of drug delivery systems or for the study of protein function in a controlled manner.
Used in Biochemical and Biotechnology Research:
N-succinimidyl-3-(2-pyridyldithio)butyrate is used as a versatile tool in biochemical and biotechnology research. Its ability to form stable yet cleavable bonds makes it an indispensable reagent for a wide range of experiments, from the development of biosensors to the study of enzyme mechanisms.

Check Digit Verification of cas no

The CAS Registry Mumber 107348-47-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,7,3,4 and 8 respectively; the second part has 2 digits, 4 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 107348-47:
(8*1)+(7*0)+(6*7)+(5*3)+(4*4)+(3*8)+(2*4)+(1*7)=120
120 % 10 = 0
So 107348-47-0 is a valid CAS Registry Number.
InChI:InChI=1/C13H14N2O4S2/c1-9(20-21-10-4-2-3-7-14-10)8-13(18)19-15-11(16)5-6-12(15)17/h2-4,7,9H,5-6,8H2,1H3

107348-47-0SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 16, 2017

Revision Date: Aug 16, 2017

1.Identification

1.1 GHS Product identifier

Product name 3-[[1-(2,5-dioxopyrrolidin-1-yl)-2H-pyridin-2-yl]disulfanyl]butanoate

1.2 Other means of identification

Product number -
Other names -

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
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More Details:107348-47-0 SDS

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