1076232-13-7Relevant academic research and scientific papers
An engineered aryl azide ligase for site-specific mapping of protein-protein interactions through photo-cross-linking
Baruah, Hemanta,Puthenveetil, Sujiet,Choi, Yoon-Aa,Shah, Samit,Ting, Alice Y.
, p. 7018 - 7021 (2008)
(Chemical Equation Presented) Labeled and linked: The small-molecule binding site of Escherichia coli lipoic acid ligase was re-engineered to accept a fluorinated aryl azide probe in place of lipoic acid. Labeling with this mutant is highly specific for LAP fusion proteins. In cell lysate, FK506 binding protein was labeled and rapamycin-dependent photocross-linking to its interaction partner was demonstrated.
