108321-42-2 Usage
Uses
Used in Molecular Biology:
Geneticin is used as a selection agent in molecular biology for the selection and maintenance of prokaryotic and eukaryotic cells. The optimal concentrations for selection and maintenance vary by cell lines, with bacteria and algae requiring 5 μg/mL or less, and animal cells requiring 300-500 μg/mL.
Used in Genetic Engineering:
Geneticin is used in genetic engineering to select for cells that have been successfully transformed with a specific gene of interest. Resistance to Geneticin is conferred by the neo gene from Tn5, which encodes an aminoglycoside 3'-phosphotransferase, APH 3'II.
Used in Research:
Geneticin is used in research to study the effects of the antibiotic on various cell types and to develop methods for selecting genetically modified cells.
Used in Drug Development:
Geneticin is used in drug development as a membrane-permeant allosteric effector of hemoglobin, which has therapeutic potential in cardiovascular diseases and cancer.
Biochem/physiol Actions
Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.
Mode of action
Geneticin works by blocking polypeptide synthesis by preventing the elongation step within eukaryotic cells.Resistance: Resistance to Geneticin is conferred through the NPTll gene in plant cells and the NEO gene in mammalian cells, which encodes aminoglycoside 3'-phosphotransferase.Action: Geneticin functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.
references
[1] bar-nun s, shneyour y, beckmann js. g-418, an elongation inhibitor of 80 s ribosomes. biochim biophys acta. 1983, 741 (1): 123-7.[2] julian davies and antonio jimenez. a new selective agent for eukaryotic cloning vectors. am. j. trop. med. hyg. 1980, 29 (5): 1089-1092.[3] xianchao g. zhang, peter w. mason, edward j. dubovi, xiaodong xu, nigel bourne, randall w. renshaw, timothy m. block, and alexander v. birk. antiviral activity of geneticin against dengue virus. antiviral res. 2009 july, 83(1): 21–27.
Check Digit Verification of cas no
The CAS Registry Mumber 108321-42-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,8,3,2 and 1 respectively; the second part has 2 digits, 4 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 108321-42:
(8*1)+(7*0)+(6*8)+(5*3)+(4*2)+(3*1)+(2*4)+(1*2)=92
92 % 10 = 2
So 108321-42-2 is a valid CAS Registry Number.
InChI:InChI=1/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9-,10-,11+,12-,13-,14-,15+,16?,17-,18-,19?,20+;;/m1../s1
