1093348-41-4Relevant academic research and scientific papers
Efficient one-cycle affinity selection of binding proteins or peptides specific for a small-molecule using a T7 phage display pool
Takakusagi, Yoichi,Kuramochi, Kouji,Takagi, Manami,Kusayanagi, Tomoe,Manita, Daisuke,Ozawa, Hiroko,Iwakiri, Kanako,Takakusagi, Kaori,Miyano, Yuka,Nakazaki, Atsuo,Kobayashi, Susumu,Sugawara, Fumio,Sakaguchi, Kengo
, p. 9837 - 9846 (2008)
Here, we report an efficient one-cycle affinity selection using a natural-protein or random-peptide T7 phage pool for identification of binding proteins or peptides specific for small-molecules. The screening procedure involved a cuvette type 27-MHz quartz-crystal microbalance (QCM) apparatus with introduction of self-assembled monolayer (SAM) for a specific small-molecule immobilization on the gold electrode surface of a sensor chip. Using this apparatus, we attempted an affinity selection of proteins or peptides against synthetic ligand for FK506-binding protein (SLF) or irinotecan (Iri, CPT-11). An affinity selection using SLF-SAM and a natural-protein T7 phage pool successfully detected FK506-binding protein 12 (FKBP12)-displaying T7 phage after an interaction time of only 10 min. Extensive exploration of time-consuming wash and/or elution conditions together with several rounds of selection was not required. Furthermore, in the selection using a 15-mer random-peptide T7 phage pool and subsequent analysis utilizing receptor ligand contact (RELIC) software, a subset of SLF-selected peptides clearly pinpointed several amino-acid residues within the binding site of FKBP12. Likewise, a subset of Iri-selected peptides pinpointed part of the positive amino-acid region of residues from the Iri-binding site of the well-known direct targets, acetylcholinesterase (AChE) and carboxylesterase (CE). Our findings demonstrate the effectiveness of this method and general applicability for a wide range of small-molecules.
