1096058-76-2Relevant academic research and scientific papers
A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells
Yatzeck, Melissa M.,Lavis, Luke D.,Chao, Tzu-Yuan,Chandran, Sunil S.,Raines, Ronald T.
supporting information; experimental part, p. 5864 - 5866 (2009/06/25)
A derivative of rhodamine 110 has been designed and assessed as a probe for cytochrome P450 activity. This probe is the first to utilize a 'trimethyl lock' that is triggered by cleavage of an ether bond. In vitro, fluorescence was manifested by the CYP1A1 isozyme with kcat/KM = 8.8 × 103 M-1 s-1 and KM = 0.09 μM. In cellulo, the probe revealed the induction of cytochrome P450 activity by the carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin, and its repression by the chemoprotectant resveratrol.
