109653-47-6Relevant articles and documents
Preparation method and application of targeting mitochondrial fluorescein derivative fluorescent probe
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Paragraph 0034-0036; 0040-0042; 0046-00480052-0054, (2021/06/06)
The invention discloses a preparation method of a mitochondrial targeting fluorescein derivative fluorescent probe, and belongs to the technical field of biology. The method comprises the steps: making fluorescein and hydrazine hydrate react to obtain a fluorescein hydrazine compound; adding the fluorescein hydrazine compound into an amino acid absolute ethyl alcohol solution to react to obtain a fluorescein hydrazine derivative; reacting the fluorescein hydrazine derivative with a mitochondrial targeting ligand to obtain a fluorescein hydrazine derivative-mitochondrial targeting substance; and reacting the fluorescein hydrazine derivative-mitochondrial targeting substance with recognition molecules to obtain the targeting mitochondrial fluorescein derivative fluorescent probe. The targeting mitochondrial fluorescein derivative fluorescent probe obtained by the invention has high recognizability on thiophenol and strong anti-interference detection capability; the positioning effect on the mitochondria is good, and the Pearson coefficient obtained by detecting the mitochondria is 0.96; and the mitochondrial membrane potential is obviously reduced.