109959-07-1Relevant academic research and scientific papers
Synthesis of the milk oligosaccharide 2′-fucosyllactose using recombinant bacterial enzymes
Albermann, Christoph,Piepersberg, Wolfgang,Wehmeier, Udo F
, p. 97 - 103 (2001)
The enzymatic synthesis of GDP-β-L-fucose and its enzymatic transfer reaction using recombinant enzymes from bacterial sources was examined. The GDP-D-mannose 4,6-dehydratase and the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase from Escherichia coli K-12, respectively, were used to catalyse the conversion of GDP-α-D-mannose to GDP-β-L-fucose with 78% yield. For the transfer of the L-fucose to an acceptor, we cloned and overproduced the α-(1 → 2)-fucosyltransferase (FucT2) protein from Helicobacter pylori. We were able to synthesise 2′-fucosyllactose using the overproduced FucT2 enzyme, enzymatically synthesised GDP-L-fucose and lactose. The isolation of 2′-fucosyllactose was accomplished by anion-exchange chromatography and gel filtration to give 65% yield.
