1100-22-7

Usage

Uses

Used in Biochemical Research:
DANSYL-L-LEUCINE is used as a fluorescent probe for studying enzyme kinetics, protein folding, and peptide binding due to its strong fluorescence emission upon ultraviolet light excitation.
Used in Enzyme Kinetics Studies:
DANSYL-L-LEUCINE is used as a substrate analog to monitor enzyme activities, providing insights into the catalytic mechanisms and reaction rates of enzymes that act on leucine-containing peptides.
Used in Protein Folding Studies:
DANSYL-L-LEUCINE is used as a spectroscopic probe to investigate the conformational changes in proteins during the folding process, offering a means to study the thermodynamics and kinetics of protein folding.
Used in Peptide Binding Studies:
DANSYL-L-LEUCINE is used as a ligand to explore the binding interactions between peptides and their target proteins or enzymes, facilitating the understanding of molecular recognition and binding mechanisms in biological systems.

Upstream product

• 65452-14-4 dansyl-D,L-leucine 

Relevant academic research and scientific papers

Preparation and evaluation of a triazole-bridged bis(β-cyclodextrin)–bonded chiral stationary phase for HPLC

A triazole-bridged bis(β-cyclodextrin) was synthesized via a high-yield Click Chemistry reaction between 6-azido-β-cyclodextrin and 6-propynylamino-β-cyclodextrin, and then it was bonded onto ordered silica gel SBA-15 to obtain a novel triazole-bridged bis (β-cyclodextrin)–bonded chiral stationary phase (TBCDP). The structures of the bridged cyclodextrin and TBCDP were characterized by the infrared spectroscopy, mass spectrometry, elemental analysis, and thermogravimetric analysis. The chiral performance of TBCDP was evaluated by using chiral pesticides and drugs as probes including triazoles, flavanones, dansyl amino acids and β-blockers. Some effects of the composition in mobile phase and pH value on the enantioseparations were investigated in different modes. The nine triazoles, eight flavanones, and eight dansyl amino acids were successfully resolved on TBCDP under the reversed phase with the resolutions of hexaconazole, 2′-hydroxyflavanone, and dansyl-DL-tyrosine, which were 2.49, 5.40, and 3.25 within 30 minutes, respectively. The ten β-blockers were also separated under the polar organic mode with the resolution of arotinolol reached 1.71. Some related separation mechanisms were discussed preliminary. Compared with the native cyclodextrin stationary phase (CDSP), TBCDP has higher enantioselectivity to separate more analytes, which benefited from the synergistic inclusion ability of the two adjacent cavities and bridging linker of TBCDP, thereby enabling it a promising prospect in chiral drugs and food analysis.

Cationic β-cyclodextrin-modified hybrid magnetic microspheres as chiral selectors for selective chiral absorption of dansyl amino acids

Chirally selective functionalized magnetic microspheres show great potential in enantiomeric separations. In this study, a novel class of chiral magnetic selectors was developed by immobilization of vinylimidazolium-β- cyclodextrin chloride (VIMCD-Cl) on 3-methacryloxypropyltrimethoxysilane- modified iron oxide magnetic microspheres through a radical polymerization. The prepared chiral materials have regular three-dimensional core-shell architectures with an average particle size of about 580 nm and a high magnetization saturation of about 51 emu g-1. Fourier transform-infrared spectra (FT-IR), thermogravimetric analysis (TGA) and elemental analysis confirmed that VIMCD-Cl was successfully polymerized on the surface of the magnetic microspheres. The prepared functional magnetic materials were then applied in the selective chiral absorption of three dansyl amino acids using microbatch technology. The results indicated that VIMCD-Cl immobilized magnetic microspheres (VIMCD-MNPs) possessed good enantioselectivities toward the three dansyl amino acids, and showed stronger interactions with the l-enantiomers during the chiral adsorption process. Furthermore, these functionalized chiral magnetic materials possess an excellent recyclability and can be used as effective chiral magnetic selectors for chiral separations.

Chiral separation of enantiomers of amino acid derivatives by HPLC on vancomycin and teicoplanin chiral stationary phases

The chiral separation of α-amino acids by liquid chromatography using macrocyclic antibiotic bonded stationary phases were studied. Teicoplanin and vancomycin bonded stationary phases have been used to separate enantiomers of dansyl amino acids in the reversed - phase high performance liquid chromatography mode. By comparison of chromatographic parameters obtained by use of both chiral stationary phases it the mechanism of chiral separation could be suggested. The better separation - greater value of R(j,i) of enantiomers - was achieved by the teicoplanin column.

Direct resolution of optically active isomers on chiral packings containing ergoline skeletons. 5. Enantioseparation of amino acid derivatives

A new procedure for ergot alkaloid-based chiral stationary phase preparation is described. Synthesis is based on bonding the allyl derivative of terguride to mercaptopropylsilanized silica gel. The packing exhibits higher content of chiral selector, stability, reproducibility, and enantioselectivity toward amino acids compared to that previously studied. The chromatographic behavior of amino acids with different side chains and substituent groups is investigated in order to obtain a deeper insight into the enantiodiscriminative mechanism as well as to determine the limitations and strengths of terguride as a chiral selector for this class of compounds. A variety of factors, including mobile phase parameters such as pH, ionic strength, content and nature of organic modifier, and temperature, are examined. A new procedure for ergot alkaloid-based chiral stationary phase preparation is described. Synthesis is based on bonding the allyl derivative of terguride to mercaptopropylsilanized silica gel. The packing exhibits higher content of chiral selector, stability, reproducibility, and enantioselectivity toward amino acids compared to that previously studied. The chromatographic behavior of amino acids with different side chains and substituent groups is investigated in order to obtain a deeper insight into the enantiodiscriminative mechanism as well as to determine the limitations and strengths of terguride as a chiral selector for this class of compounds. A variety of factors, including mobile phase parameters such as pH, ionic strength, content and nature of organic modifier, and temperature, are examined.