11021-13-9Relevant articles and documents
Purification and characterization of ginsenoside Ra-hydrolyzing beta-D-xylosidase from Bifidobacterium breve K-110, a human intestinal anaerobic bacterium.
Shin, Ho-Young,Lee, Ji-Hyun,Lee, Jang-Yeon,Han, Yeo-Ock,Han, Myung Joo,Kim, Dong-Hyun
, p. 1170 - 1173 (2003)
Beta-D-Xylosidase (EC 3.2.1.37) has been purified from ginsenoside Ra-metabolizing Bifidobacterium breve K-110, which was isolated from human intestinal microflora. beta-D-Xylosidase was purified to apparent homogeneity by a combination of ammonium sulfate precipitation, QAE-cellulose, butyl-toyopearl, hydroxyapatit and Q-Sepharose column chromatographies with the final specific activity of 51.8 micromol/min/mg. Molecular weight of beta-D-xylosidase is 49 kDa by SDS-PAGE and gel filtration, which consisted of a single subunit. beta-D-Xylosidase showed optimal activity at pH 5.0 and 37 degrees C. The purified enzyme was potently inhibited by PCMS. beta-D-Xylosidase acted to the greatest extent on p-nitrophenyl-beta-D-xylopyranoside, followed by ginsenoside Ra1 and ginsenoside Ra2. This enzyme hydrolyzed xylan to xylose, but did not act on p-nitrophenyl-beta-glucopyranoside, p-nitrophenyl-beta-galactopyranoside or p-nitrophenyl-beta-D-fucopyranoside. These findings suggest that this is the first reported purification of ginsenoside-hydrolyzing beta-D-xylosidase from an anaerobic Bifidobacterium sp.
Acylated protopanaxadiol-type ginsenosides from the root of Panax ginseng
Zhu, Guo-Yuan,Li, Ying-Wei,Kwok-Po Hau, Desmond,Jiang, Zhi-Hong,Yu, Zhi-Ling,Fong, Wang-Fun
experimental part, p. 1853 - 1863 (2012/01/13)
Six new protopanaxadiol-type ginsenosides, named ginsenosides Ra 4-Ra9 (1-6, resp.), along with 14 known dammarane-type triterpene saponins, were isolated from the root of Panax ginseng, one of the most important Chinese medicinal herbs. The structures of the new compounds were determined by spectroscopic methods, including 1D- and 2D-NMR, HR-MS, and chemical transformation as (20S)- 3-O-{β-D-6-O-[(E)-but-2-enoyl] glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[β-D-xylopyranosyl- (1→4)-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyl] protopanaxadiol (1), (20S)-3-O-[β-D-6-O-acetylglucopyranosyl-(1→2)- β-D-glucopyranosyl]-20-O-[β-D-xylopyranosyl-(1→4) -α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (2), (20S)-3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β- D-glucopyranosyl}-20-O-[β-D-glucopyranosyl-(1→6)-β-D- glucopyranosyl]protopanaxadiol (3), (20S)-3-O-{β-D-6-O-[(E)-but-2-enoyl] glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[α-L- arabinopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (4), (20S)-3-O-{β-D-4-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2) -β-D-glucopyranosyl}-20-O-[α-L-arabinofuranosyl-(1→6) -β-D-glucopyranosyl]protopanaxadiol (5), (20S)-3-O-{β-D-6-O-[(E)-but- 2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[α-L- arabinofuranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (6). The sugar moiety at C(3) of the aglycone of each new ginsenoside is butenoylated or acetylated. Copyright
Chemical studies on crude drug processing. VI. (1)) Chemical structures of malonyl-Ginsenosides Rb1, Rb2, Rc, and Rd isolated from the root of Panax ginseng C.A. Meyer
Kitagawa,Taniyama,Yoshikawa,Ikenishi,Nakagawa
, p. 2961 - 2970 (2007/10/02)
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