111072-31-2 Usage
Uses
Different sources of media describe the Uses of 111072-31-2 differently. You can refer to the following data:
1. Tetrazolium derivative useful in cancer research.XTT sodium salt is used in conjunction with phenazine methosulfate to screen human cancer cell lines. It is also used to study fungal cell damage. It is involved in testing antimicrobial susceptibility of staphylococci and in candida biofilm research. Further, it is used in an assay for cell viability and cell proliferation.
2. Useful in AIDS and cancer testing.
General Description
XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.
Biochem/physiol Actions
XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.
Check Digit Verification of cas no
The CAS Registry Mumber 111072-31-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,1,0,7 and 2 respectively; the second part has 2 digits, 3 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 111072-31:
(8*1)+(7*1)+(6*1)+(5*0)+(4*7)+(3*2)+(2*3)+(1*1)=62
62 % 10 = 2
So 111072-31-2 is a valid CAS Registry Number.
InChI:InChI=1/C22H19N7O13S2.2Na.H2O/c1-41-18-8-16(28(31)32)20(43(35,36)37)10-14(18)26-12-25(22(30)23-13-6-4-3-5-7-13)24-27(26)15-11-21(44(38,39)40)17(29(33)34)9-19(15)42-2;;;/h3-12H,1-2H3,(H,23,30)(H,35,36,37)(H,38,39,40);;;1H2/q+2;2*+1;/p-2
111072-31-2Relevant articles and documents
The Synthesis of XTT: A New Tetrazolium Reagent that is Bioreducible to a Water-Soluble Formazan
Paull, Keneth D.,Shoemaker, Robert H.,Boyd, Michael R.,Parsons, Jack L.,Risbood, Prabhakar A.,et al.
, p. 911 - 914 (2007/10/02)
A new tetrazolium salt, XTT, has been synthesized.XTT is reduced by a considerable variety of cell lines to a water-soluble formazan.XTT appears to merit further investigation as a reagent for broader application to cell culture assay systems.
