112806-33-4 Usage
Uses
Used in Biological Studies:
Amitriptyline N-glucuronide is used as a research tool for studying the biphasic kinetics of quaternary ammonium glucuronide formation from amitriptyline and diphenhydramine in human liver microsomes. This helps researchers understand the metabolic pathways and enzyme activities involved in the glucuronidation process.
Used in Analytical Studies:
Amitriptyline N-glucuronide is used as a target analyte in analytical studies to detect the presence of amitriptyline and diphenhydramine N-glucuronides in human urine samples. High-performance liquid chromatography (HPLC) techniques, with and without hydrolysis, are employed to accurately measure and quantify these metabolites, providing valuable information on drug exposure, metabolism, and potential drug-drug interactions.
Check Digit Verification of cas no
The CAS Registry Mumber 112806-33-4 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,2,8,0 and 6 respectively; the second part has 2 digits, 3 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 112806-33:
(8*1)+(7*1)+(6*2)+(5*8)+(4*0)+(3*6)+(2*3)+(1*3)=94
94 % 10 = 4
So 112806-33-4 is a valid CAS Registry Number.
InChI:InChI=1/C26H31NO6/c1-27(2,25-23(30)21(28)22(29)24(33-25)26(31)32)15-7-12-20-18-10-5-3-8-16(18)13-14-17-9-4-6-11-19(17)20/h3-6,8-12,21-25,28-30H,7,13-15H2,1-2H3/t21-,22-,23+,24-,25+/m0/s1
112806-33-4Relevant academic research and scientific papers
A convenient new synthesis of quaternary ammonium glucuronides of drug molecules
Iddon, Lisa,Bragg, Ryan A.,Harding, John R.,Stachulski, Andrew V.
experimental part, p. 537 - 541 (2010/03/03)
N-Glucuronides, of various structural types, are frequently encountered as drug metabolites. Efficient chemical synthesis of these compounds, both as analytical standards and for toxicological investigation, is therefore an important goal. Earlier syntheses of N+- glucuronides of aliphatic tertiary amine drugs involved direct reaction of the drug molecule with a bromosugar, but yields were generally low and of poor reproducibility, with many by-products. In addition the final products were often of low stability, hindering effective isolation and purification. We now report that a stable, readily prepared glucuronic acid hemiacetal is a reliable precursor for metabolites of this type and give three pharmaceutically relevant examples. We report further on the stability of the final metabolites and the conditions required for their isolation and purification.
