114162-64-0

Basic information

• Product Name: 5-BROMO-4-CHLORO-3-INDOLYL B-D-
• Synonyms: 5-BroMo-4-chloro-1H-indol-3-yl β-D-Glucopyranosiduronic Acid MonosodiuM Salt;5-Bromo-4-chloro-3-indolyl beta-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt X-Gluc CHA Salt X-GlcA CHA Salt;X-gluc/X-GlcA;5-BroMo-4-chloro-3-indolyl β-D-Glucuronide CyclohexylaMMoniuM Salt [for BiocheMical Research];5-BroMo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylaMMoniuM salt hydrate (X-GLUC);5-broMo-4-chloro-1H-indol-3-yl, coMpd. with cyclohexanaMine (1:1);5-BroMo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylaMMoniuM salt Monohydrate;CyclohexanaMine (2S,3S,4S,5R,6S)-6-((5-broMo-4-chloro-1H-indol-3-yl)oxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylate
• CAS NO: 114162-64-0
• Molecular Formula: C14H13BrClNO7.C6H13N
• Molecular Weight: 521.789
• EINECS: 1533716-785-6
• Product Categories: Carbohydrates & Derivatives;Fluorescent Labels & Indicators;Glucuronides;Labeling and Diagnostics Reagents;substrate
• Mol File: 114162-64-0.mol
• Article Data: 0

Chemical Properties

• Melting Point: 228-230°C
• Boiling Point: 732.2 °C at 760 mmHg
• Flash Point: 396.6 °C
• Appearance: White to off-white or colorless/tablet
• Density: 1.4241 (rough estimate)
• Vapor Pressure: 8.69E-23mmHg at 25°C
• Refractive Index: 1.6110 (estimate)
• Storage Temp.: 2-8°C
• Solubility: DMF: soluble
• Stability: Hygroscopic
• CAS DataBase Reference: 5-BROMO-4-CHLORO-3-INDOLYL B-D-(CAS DataBase Reference)
• NIST Chemistry Reference: 5-BROMO-4-CHLORO-3-INDOLYL B-D-(114162-64-0)
• EPA Substance Registry System: 5-BROMO-4-CHLORO-3-INDOLYL B-D-(114162-64-0)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-37/39-24/25
• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 114162-64-0(Hazardous Substances Data)

Usage

Chemical Properties

White Solid

Uses

Different sources of media describe the Uses of 114162-64-0 differently. You can refer to the following data:
1. 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining.
2. Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.X-GlcA has been used in histochemical staining of root sections for light microscopic observation.

General Description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by?Escherichia coli.

InChI:InChI=1/C18H21BrClNO7.C6H13N/c1-15(24)13(14(22)23)28-18(4,17(3,26)16(15,2)25)27-10-7-21-9-6-5-8(19)12(20)11(9)10;7-6-4-2-1-3-5-6/h5-7,13,21,24-26H,1-4H3,(H,22,23);6H,1-5,7H2/t13?,15-,16u,17u,18-;/m1./s1