1151515-75-1Relevant academic research and scientific papers
A semisynthetic fluorescent sensor protein for glutamate
Brun, Matthias A.,Tan, Kui-Thong,Griss, Rudolf,Kielkowska, Anna,Reymond, Luc,Johnsson, Kai
, p. 7676 - 7678 (2012/07/02)
We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor displays a ratiometric change of 1.56. The high sensitivity toward glutamate concentration changes of the sensor and its exclusive extracellular localization make it an attractive tool for glutamate sensing in neurobiology.
Semisynthetic fluorescent sensor proteins based on self-labeling proteintags
Brun, Matthias A.,Tan, Kui-Thong,Nakata, Eiji,Hinner, Marlon J.,Johnsson, Kai
supporting information; experimental part, p. 5873 - 5884 (2009/09/24)
Genetically encoded fluorescent sensor proteins offer the possibility toprobe the concentration of key metabolites in living cells. The approac hes currently used to generate such fluorescent sensor proteins lack generality, as they require a protein that
