115416-39-2Relevant academic research and scientific papers
Stereochemistry of Cysteinsulphinic Acid Decarboxylase
Aberhart, D. John
, p. 1547 - 1550 (2007/10/02)
An h.p.l.c.-based assay for cysteinsulphinic acid decarboxylase has been developed.The assay simultaneously measures glutamate decarboxylation and/or taurine formation.Using the cysteinesulphinic acid decarboxylase in rat liver homogenate, L-cysteinsulphinic acid was converted into hypotaurine.Similarly, unlabelled L-cysteinsulphinic acid was transformed by rat liver homogenate in a D2O-containing medium into the enantiomeric hypotaurine.The products were desulphurized by treatment with Raney nickel, and the resulting enantiomeric ethylamines converted into the corresponding (-)-camphanamides.Deuterium n.m.r. spectra of the latter revealed that the decarboxylations proceed stereospecifically with retention of configuration.
