1159680-46-2Relevant academic research and scientific papers
Preparation of (2?-5?)-oligonucleotides based on 6-N- benzylaminopurineriboside using the Spicaria violacea fungal enzyme complex
Kulak,Tkachenko,Grishan,Kukharskaya,Eroshevskaya,Kalinichenko,Zinchenko
experimental part, p. 74 - 78 (2009/07/18)
A method for chemico-enzymatic synthesis of (2?-5?)-oligonucleotides with 6-N-benzylaminopurineriboside as the nucleoside units was proposed. The method consisted of enzymatic hydrolysis of the oligonucleotides with mixed (2?-5?)-(3?-5?)-phosphodiesterbonds that were prepared by polymerization of 6-N-benzyladenosine-2?(3?)-monophosphate by using (3?-5?)-specific nuclease and phosphatase contained in the filtrate of culture medium of the mycelial fungus Spicaria violacea.
