117138-06-4Relevant academic research and scientific papers
Stereochemical analysis of isopentenyl diphosphate isomerase type II from Staphylococcus aureus using chemically synthesized (S)- and (R)-[2- 2H]Isopentenyl diphosphates
Kao, Chai-Lin,Kittleman, William,Zhang, Hua,Seto, Haruo,Liu, Hung-Wen
, p. 5677 - 5680 (2005)
(Chemical Equation Presented) To study the catalysis of isopentenyl diphosphate (IPP) isomerase type II from Staphylococcus aureus, which is a flavoprotein catalyzing the interconversion of IPP and dimethylallyl diphosphate, we have chemically synthesized (S)- and (R)-[2-2H]IPP and carried out stereochemical analysis of the reaction. Our results show that the C-2 deprotonation of IPP by this enzyme is pro-R stereospecific, suggesting a similar stereochemical course as the type I enzyme.
