1173671-63-0

Basic information

• Product Name: D-Tryptophan (2E)-2-(6-quinoxalinylmethylene)hydrazide
• Synonyms: D-Tryptophan (2E)-2-(6-quinoxalinylmethylene)hydrazide;Rhosin;Rhosin /G04, (2R)-2-Amino-3-(1H-indol-3-yl)-N′-((1E)-quinoxalin-6-ylmethylidene)propanehydrazide
• CAS NO: 1173671-63-0
• Molecular Formula: C20H18N6O
• Molecular Weight: 358.39652
• Mol File: 1173671-63-0.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Density: 1.38±0.1 g/cm3(Predicted)
• Storage Temp.: +2C to +8C
• PKA: 12.00±0.46(Predicted)
• CAS DataBase Reference: D-Tryptophan (2E)-2-(6-quinoxalinylmethylene)hydrazide(CAS DataBase Reference)
• NIST Chemistry Reference: D-Tryptophan (2E)-2-(6-quinoxalinylmethylene)hydrazide(1173671-63-0)
• EPA Substance Registry System: D-Tryptophan (2E)-2-(6-quinoxalinylmethylene)hydrazide(1173671-63-0)

Safety Data

• Hazardous Substances Data: 1173671-63-0(Hazardous Substances Data)

Usage

General Description

A cell-permeable (quinoxalinylmethylidene-D-tryptophanyl)hydrazide that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs (including LARG, DBL, LBC, p115 RhoGEF, and PDZ RhoGEF), but not p190RhoGAP, RhoGDI, or its effectors (ROCK, mDia, PKN, Rhoteckin). Shown to suppress mammospheres formation in MCF7, but not the non-tumorigenic MCF10A, cultures and inhibit serum-induced RhoA, RhoB, and RhoC, but not Cdc42 or Rac1, activation, MLC & PAK phosphorylation, as well as stress fiber and focal complex formation in serum-starved NIH3T3 cultures (Effective conc. 30 μM) in a reversible manner. RhoA inactivation by G04 treatment (30 μM) is also demonstrated to promote neurite outgrowth and branching from NGF-treated PC12 cells.

Biochem/physiol Actions

Primary TargetRho GTPase