1174518-50-3Relevant academic research and scientific papers
Salinipeptins: Integrated Genomic and Chemical Approaches Reveal Unusual d -Amino Acid-Containing Ribosomally Synthesized and Post-Translationally Modified Peptides (RiPPs) from a Great Salt Lake Streptomyces sp.
Shang, Zhuo,Winter, Jaclyn M.,Kauffman, Christopher A.,Yang, Inho,Fenical, William
, p. 415 - 425 (2019)
Analysis of the full genome of an environmentally unique, halotolerant Streptomyces sp. strain GSL-6C, isolated from the Great Salt Lake, revealed a gene cluster encoding the biosynthesis of the salinipeptins, d-amino-acid-containing members of the rare linaridin subfamily of ribosomally synthesized and post-translationally modified peptides (RiPPs). The sequence organization of the unmodified amino acid residues in salinipeptins A-D (1-4) were suggested by genome annotation, and subsequently, their sequence and post-translational modifications were defined using a range of spectroscopic techniques and chemical derivatization approaches. The salinipeptins are unprecedented linaridins bearing nine d-amino acids, which are uncommon in RiPP natural products and are the first reported in the linaridin subfamily. Whole genome mining of GSL-6C did not reveal any homologues of the reported genes responsible for amino acid epimerization in RiPPs, inferring new epimerases may be involved in the conversion of l- to d-amino acids. In addition, the N-oxide and dimethylimidazolidin-4-one moieties in salinipeptins B and C, which are modified from N,N-dimethylalanine, are unknown in bacterial peptides. The three-dimensional structure of salinipeptin A, possessing four loops generated by significant hydrogen bonding, was established on the basis of observed nuclear Overhauser effect (NOE) correlations. This study demonstrates that integration of genomic information early in chemical analysis significantly facilitates the discovery and structure characterization of novel microbial secondary metabolites.
Synthesis of poly (γ-glutamic acid) and heterologous expression of pgsBCA genes
Cao, Mingfeng,Song, Cunjiang,Jin, Yinghong,Liu, Li,Liu, Jing,Xie, Hui,Guo, Wenbin,Wang, Shufang
experimental part, p. 111 - 116 (2011/02/23)
The genes required for synthesis of poly (γ-glutamic acid) (γ-PGA) were cloned from Bacillus licheniformis NK-03, a strain isolated from fermented food, natto. There were three open reading frames pgsB, pgsC, pgsA in the cloned fragment, all of which were greatly similar with those from typical Bacillus subtilis strains. The alignment of deduced amino acid sequences showed that PgsC was the most conservative part in PgsBCA. Recombinant plasmid pXMJ19-PGS was constructed by a shuttle vector pXMJ19, and it was successfully transformed and expressed in the recombinant strains of Escherichia coli JM109 and Corynebacterium glutamicum ATCC13032, respectively. Expression of pgsBCA in C. glutamicum indicated that it could synthesize γ-PGA with a yield of 0.69 g/L and 97% proportion of l-glutamate monomer in the absence of glutamic acid. The results suggest that γ-PGA biosynthesis directly from glucose by genetic engineering is feasible and significant.
