117823-40-2Relevant academic research and scientific papers
Synthesis of methionine containing peptides related to native chemical ligation
Pachamuthu, Kandasamy,Schmidt, Richard R.
, p. 659 - 662 (2003)
Methionine chemical ligation is based on a convenient direct thioester formation at the C-terminus of the ligation site, on homocysteine reduction with dithiothreitol in order to liberate the mercapto group of the homocysteine residue at the N-terminus, and on chemoselective S-methylation with methyl iodide.
Substrate-directed lewis-acid catalysis for peptide synthesis
Muramatsu, Wataru,Hattori, Tomohiro,Yamamoto, Hisashi
supporting information, p. 12288 - 12295 (2019/08/20)
A Lewis-acid-catalyzed method for the substrate-directed formation of peptide bonds has been developed, and this powerful approach is utilized for the new "remote" activation of carboxyl groups under solvent-free conditions. The presented method has the following advantages: (1) the high-yielding peptide synthesis uses a tantalum catalyst for any amino acids; (2) the reaction proceeds without any racemization; (3) the new substrate-directed chemical ligation using the titanium catalyst is applicable to convergent peptide synthesis. These advantages overcome some of the unresolved problems in classical peptide synthesis.
CHROMOGENIC SUBSTRATES FOR PAPAIN WITH A C-TERMINAL S-BENZYLCYSTEINE RESIDUE
Kasafirek, Evzen,Fukal, Ladislav,Kas, Jan
, p. 3197 - 3205 (2007/10/02)
Two types of chromogenic substrates for papain were prepared, namely p-nitroanilides of N-acetyltripeptides of general formula Ac-Leu-Leu-A-NAn, where A = Gly, Ala, Leu, and p-nitroanilides of 3-carboxypropionyldi- and tripeptides of general formula Suc-B-Cys(Bzl)-NAn, where B = Gly, Gly-Gly, Ala, Ala-Ala, Leu, Leu-Leu, Gly-Leu, Leu-Ala and Ala-Met.The values of Km, kcat and C (kcat/Km) for these substrates were determined.Suc-Leu-Leu-Cys(Bzl)-NAn is the best substrate for papain its C being 60 000 mol-1 l s-1.
