117923-92-9

Upstream product

• 18162-48-6 tert-butyldimethylsilyl chloride 
• 117923-91-8 5'-O-dimethoxytrityl-N²,N⁶-diacetyl-2-aminoadenosine 
• 2096-10-8 adenosine-2-amine 
• 117923-90-7 N²,N⁶-diacetyl-2-aminoadenosine 
• 40615-36-9 4,4'-dimethoxytrityl chloride 

Relevant academic research and scientific papers

Evaluation of the 2 NH2A-T Pair in Hybridization, I. Synthesis of the DNA/RNA Hybrid Oligomers Containing 2-Amninoadenosines

DNA decamers containing 2-aminoadenosine were synthesized.Oligonucleotide duplexes including the 2 NH2A-T base pairs were prepared and their Tm profile examined.Contrary to expectation, elevation of the Tm value by the 2 NH2 group is very small in DNA/RNA duplexes.From the CD spectra measurement, we assume that the distortion of the B-DNA structure caused by scattered DNA/RNA base pairing diminishes the efficient hydrogen bonding and base stacking of the duplexes.It was also found that the DNA duplexes containing 2-aminoadenosine hybrids are considerably resistant to ribonuclease T2 or nuclease P1 digestion. - Keywords: DNA Probe, Hybridization, 2-Aminoadenosine, CD Spectra, DNA/RNA Hybrid Oligomer