117961-27-0

Basic information

• Product Name: N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE
• Synonyms: N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE;N-4-TOSYL-GLYCYL-L-PROLYL-L-ARGININE 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE;TOS-GLY-PRO-ARG-AMC HCL;TOSYL-GLY-PRO-ARG-AMC HCL;tosyl-glycyl-prolyl-arginyl-4-methylcoumaryl-7-amide;N-P-tosyl-gly-pro-arg 7-amido-4-*methylcoumarin H;N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-*METHYLC OUMARIN HYD;Tos-Gly-Pro-Arg-AMC
• CAS NO: 117961-27-0
• Molecular Formula: C30H38ClN7O7S
• Molecular Weight: 676.18
• Product Categories: Coagulation Proteins and ReagentsEnzyme Substrates;Plasma&Blood Proteins;Protease Substrates;Proteins and Derivatives;Substrates by Enzyme;Thrombin;ThrombinEnzyme Substrates
• Mol File: 117961-27-0.mol

Chemical Properties

• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: /
• Density: 1.46g/cm³
• Refractive Index: 1.676
• Storage Temp.: −20°C
• PKA: 9.79±0.50(Predicted)
• CAS DataBase Reference: N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE(117961-27-0)
• EPA Substance Registry System: N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE(117961-27-0)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 117961-27-0(Hazardous Substances Data)

Usage

Uses

Used in Biochemical Research:
N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is used as a substrate for the study and analysis of enzymes and peptidases, particularly serine proteases, due to its tosylated tetrapeptide structure that can be cleaved by these enzymes.
Used in Pharmaceutical Research:
In the pharmaceutical industry, N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is used as a tool for enzyme kinetics studies, which helps in understanding the mechanisms of enzyme action and the development of enzyme inhibitors.
Used in Enzyme Assays:
N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is used as a fluorescent substrate in enzyme assays to measure the activity of specific peptidases. The cleavage of the 7-amido-4-methylcoumarin moiety by these enzymes results in a measurable fluorescent signal, allowing for the quantification of enzyme activity.
Used in Drug Development:
N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is used in the development of new drugs, particularly those targeting enzymes and peptidases. Its use in enzyme assays and inhibitor screening aids in the identification and optimization of potential drug candidates.
Used in Inhibitor Screening:
N-P-TOSYL-GLY-PRO-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE is used as a part of inhibitor screening processes to identify potential enzyme inhibitors. The fluorescent signal change upon substrate cleavage can indicate the presence and effectiveness of an inhibitor, which is crucial for drug discovery and development.

InChI:InChI=1/C30H37N7O7S/c1-18-7-10-21(11-8-18)45(42,43)34-17-26(38)37-14-4-6-24(37)29(41)36-28(40)23(5-3-13-33-30(31)32)35-20-9-12-22-19(2)15-27(39)44-25(22)16-20/h7-12,15-16,23-24,34-35H,3-6,13-14,17H2,1-2H3,(H4,31,32,33)(H,36,40,41)/t23-,24-/m0/s1