2-(piperidin-4-yl)ethanesulfonaMide, commonly known as PIPES, is a zwitterionic biological buffer with the chemical formula C8H18N2O4S2. It is an organic compound used extensively in biochemistry and molecular biology as a buffering agent to maintain pH levels in cell culture media and other biological solutions. With a pKa value of 6.8, PIPES is effective at physiological pH and is capable of maintaining pH stability even at elevated temperatures. Its non-toxic nature to living cells and minimal interference with biochemical reactions make it a versatile and widely used chemical in life sciences research.
1179833-77-2
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Basic information
- Product Name: 2-(piperidin-4-yl)ethanesulfonaMide
- Synonyms: 2-(piperidin-4-yl)ethanesulfonaMide;2-(4-piperidyl)ethanesulfonaMide;4-Piperidineethanesulfonamide
- CAS NO:1179833-77-2
- Molecular Formula: C7H16N2O2S
- Molecular Weight: 192.27914
- EINECS: N/A
- Product Categories: N/A
- Mol File: 1179833-77-2.mol
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: N/A
- Solubility: N/A
- CAS DataBase Reference: 2-(piperidin-4-yl)ethanesulfonaMide(CAS DataBase Reference)
- NIST Chemistry Reference: 2-(piperidin-4-yl)ethanesulfonaMide(1179833-77-2)
- EPA Substance Registry System: 2-(piperidin-4-yl)ethanesulfonaMide(1179833-77-2)
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Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- WGK Germany:
- RTECS:
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 1179833-77-2(Hazardous Substances Data)
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1179833-77-2 Usage
Uses
Used in Biochemistry and Molecular Biology Research:PIPES is used as a buffering agent for maintaining pH levels in cell culture media and other biological solutions. Its ability to maintain pH stability at high temperatures and its non-toxicity to living cells make it an ideal choice for various research applications.
Used in Cell Culture Media Preparation:
PIPES is used as a buffering agent in the preparation of cell culture media to ensure optimal pH conditions for cell growth and proliferation. Its effectiveness at physiological pH and its non-toxic properties contribute to maintaining a stable and healthy cellular environment.
Used in Enzyme Assays and Biochemical Reactions:
PIPES is employed as a buffering agent in enzyme assays and other biochemical reactions to maintain a consistent pH, ensuring accurate and reliable results. Its minimal interference with biochemical processes makes it a preferred choice for such applications.
Used in High-Temperature Biological Applications:
Due to its ability to maintain pH stability at elevated temperatures, PIPES is used in high-temperature biological applications, such as thermal cycling in PCR or other temperature-sensitive assays, where maintaining a consistent pH is crucial for the success of the experiment.
Check Digit Verification of cas no
The CAS Registry Mumber 1179833-77-2 includes 10 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 7 digits, 1,1,7,9,8,3 and 3 respectively; the second part has 2 digits, 7 and 7 respectively.Calculate Digit Verification of CAS Registry Number 1179833-77:
(9*1)+(8*1)+(7*7)+(6*9)+(5*8)+(4*3)+(3*3)+(2*7)+(1*7)=202
202 % 10 = 2
So 1179833-77-2 is a valid CAS Registry Number.
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2-(piperidin-4-yl)ethanesulfonaMide
