119374-55-9Relevant academic research and scientific papers
ENERGY TRANSFER ASSAY METHOD AND REAGENT
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, (2008/06/13)
Disclosed is a non-fluorescent cyanine dye that may be used as an acceptor in fluorescence energy transfer assays involving the detection of binding and/or cleavage events in reactions involving biological molecules, and assay methods utilising such dyes. The non-fluorescent cyanine dye is a compound of formula (I), wherein the linker group Q contains at least one double bond and forms a conjugated system with the rings containing X and Y; groups R, R, Rand Rare attached to the rings containing X and Y, or optionally, are attached to atoms of the Zand Zring structures; Zand Zeach represent a bond or the atoms necessary to complete one or two fused aromatic rings each ring having five or six atoms, selected from carbon atoms and, optionally, no more than two oxygen, nitrogen and sulphur atoms; at least one of groups R, R, R, R, R, Rand Ris a target bonding group; any remaining groups R, R, R, Rand Rgroups are independently selected from the group consisting of hydrogen, C1-C4 alkyl, OR, COOR, nitro, amino, acylamino, quaternary ammonium, phosphate sulphonate and sulphate, where Ris selected from H and C1-C4 alkyl; any remaining Rand Rare selected from C1-C10 alkyl which may be unsubstituted or substituted with phenyl, the phenyl being optionally substituted by up to two substituents selected from carboxyl, sulphonate and nitro groups; characterised in that at least one of the groups R, R, R, R, R, Rand Rcomprises a substituent which reduces the fluorescence emission of said dye such that it is essentially non-fluorescent.
