119595-84-5Relevant academic research and scientific papers
Biosynthesis of 4-methylproline in cyanobacteria: Cloning of nosE and nosF genes and biochemical characterization of the encoded dehydrogenase and reductase activities
Luesch, Hendrik,Hoffmann, Dietmar,Hevel, Joan M.,Becker, Julia E.,Golakoti, Trimurtulu,Moore, Richard E.
, p. 83 - 91 (2007/10/03)
The biosynthesis of the unusual amino acid 4-methylproline in the Nostoc genus of cyanobacteria was investigated on the genetic and enzymatic level. Two genes involved in the biosynthesis were cloned and the corresponding enzymes, a zinc-dependent long-chain dehydrogenase and a Δ1-pyrroline-5-carboxylic acid (P5C) reductase homologue, were overexpressed in Escherichia coli and biochemically characterized. Putative substrates were synthesized to test enzyme substrate specificities, and deuterium labeling studies were carried out to reveal the stereospecificities of the enzymatic reactions with respect to the substrates as well as to the coenzymes.
Synthesis of 4-Substituted Prolines as Conformationally Constrained Amino Acid Analogues
Koskinen, Ari M. P.,Rapoport, Henry
, p. 1859 - 1866 (2007/10/02)
Anionic substitution of N-(9-(9-phenylfluorenyl))-protected glutamic acid esters proceeds without loss of optical integrity to give 4-substituted glutamic acid derivatives.The 4-methyl, propyl, cyanomethyl, and phenyl analogues have thus been prepared.Pri
