1196977-79-3Relevant academic research and scientific papers
Fluorescent conjugated polyfluorene with pendant lactopyranosyl ligands for studies of Ca2+-mediated carbohydrate-carbohydrate interaction
Chen, Qi,Cui, Yi,Zhang, Tian-Long,Cao, Jie,Han, Bao-G
experimental part, p. 13 - 19 (2010/12/20)
A well-defined fluorescent conjugated polyfluorene with pendant lactopyranosyl ligands was easily prepared through Cu(I)-catalyzed azide/alkyne click ligation and Suzuki coupling polymerization. As a fluorescent multivalent modelsystemof glycoconjugates, thepolymerwasfirstusedfor studies ofmetalion-mediatedcarbohydrate-carbohydrate interaction based on fluorescence spectroscopy. A significant fluorescence quenching of the lactosyl-bearing polyfluorene was observed upon addition of calcium ion, which is attributed to the polymer aggregation derived from Ca2+-mediated complex formation. Dynamic light scattering can also prove Ca2+-induced aggregation of the polymer based on determination of the corresponding hydrodynamic diameters. The calcium-mediated lactose-lactose interaction was reversible when treated with EDTA. In control studies, Ca2+-induced fluorescence quenching can not be observed for cellobiosyl- or galactosyl-functionalized polymer analogues, which show that specific sugar structures are critical for carbohydrate-metal complex formation.
