1200461-84-2Relevant academic research and scientific papers
5-METHOXY. 3'-OH UNBLOCKED, FAST PHOTOCLEAVABLE TERMINATING NUCLEOTIDES AND METHODS FOR NUCLEIC ACID SEQUENCING
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, (2013/03/28)
The present invention relates generally to 3'-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing an
Stereochemistry of benzylic carbon substitution coupled with ring modification of 2-nitrobenzyl groups as key determinants for fast-cleaving reversible terminators
Stupi, Brian P.,Li, Hong,Wang, Jinchun,Wu, Weidong,Morris, Sidney E.,Litosh, Vladislav A.,Muniz, Jesse,Hersh, Megan N.,Metzker, Michael L.
, p. 1724 - 1727 (2012/05/05)
A complete set of 2-nitrobenzyl-modified reversible terminators have been developed, which upon exposure to UV light generate natural hydroxymethyl nucleotides (see scheme). The combination of a stereospecific (S)-tert-butyl group (R) at the benzylic carb
