1205-88-5

Basic information

• Product Name: H-GLY-PNA
• Synonyms: N'-P-NITROPHENYLGLYCINAMIDE;GLYCINE 4-NITROANILIDE;GLYCINE-PNA;GLYCINE P-NITROANILIDE;GLYCINE-P-NITROANILINE;H-GLY-PNA;Glycine p-nitroanilide hydrochloride;Glycine 4-nitroanilide≥ 99% (HPLC)
• CAS NO: 1205-88-5
• Molecular Formula: C₈H₉N₃O₃
• Molecular Weight: 195.18
• Product Categories: Activity;Chromogenic;Enzyme Substrates
• Mol File: 1205-88-5.mol
• Article Data: 4

Chemical Properties

• Boiling Point: 451.7 °C at 760 mmHg
• Flash Point: 227 °C
• Appearance: /
• Density: 1.413 g/cm³
• Vapor Pressure: 2.37E-08mmHg at 25°C
• Refractive Index: 1.654
• Storage Temp.: −20°C
• CAS DataBase Reference: H-GLY-PNA(CAS DataBase Reference)
• NIST Chemistry Reference: H-GLY-PNA(1205-88-5)
• EPA Substance Registry System: H-GLY-PNA(1205-88-5)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 1205-88-5(Hazardous Substances Data)

Usage

Chemical Properties

Off-white crystalline powder

Uses

Different sources of media describe the Uses of 1205-88-5 differently. You can refer to the following data:
1. Substrate for glycine aminopeptidase.
2. H-Gly-pna acts as proline arylaminidase substrate, for enzyme determination in human serum. Synthesis of some amide derivatives and their biological activity.

InChI:InChI=1/C8H9N3O3/c9-5-8(12)10-6-1-3-7(4-2-6)11(13)14/h1-4H,5,9H2,(H,10,12)

Upstream product

• 17329-87-2 2-chloro-N-(4-nitro-phenyl)-acetamide 
• 100-01-6 4-nitro-aniline 
• 82518-64-7 N-(4-nitrophenyl)-α-<(tert-butoxycarbonyl)amino>acetamide 
• 14235-20-2 Nα-benzyloxycarbonyl-L-glycyl-p-nitroanilide 

Downstream Products

• 123529-87-3 ((S)-3-Methyl-1-{[(4-nitro-phenylcarbamoyl)-methyl]-carbamoyl}-butyl)-carbamic acid benzyl ester 
• 88815-47-8 Boc-L-Pro-Gly-p-nitroanilide 
• 87543-86-0 Boc-L-Asn-Gly-pNA 
• 87543-89-3 Boc-L-Gln-Gly-pNA 
• 111607-85-3 acetylleucyl-leucyl-glycine p-nitroanilide 
• 71732-39-3 leucyl-glycine p-nitroanilide 
• 85697-96-7 H-Tyr-Leu-Gly-pNA*HCl 
• 82155-55-3 Suc-Tyr-Leu-Gly-pNA 
• 85697-87-6 Boc-Tyr-Leu-Gly-pNA 
• 85718-93-0 H-Leu-Gly-pNA*HCl 
• 87543-98-4 Boc-Gly-L-Pro-L-Ala-Gly-pNA 
• 87544-00-1 Boc-Gly-L-Pro-D-Ala-Gly-pNA 
• 88815-51-4 Boc-Gly-L-Ala-L-Pro-Gly-p-nitroanilide 

Relevant articles and documents

Characterisation of the aminopeptidase from non-germinated winter rape (Brassica napus L.) seeds

Rapeseed plays a crucial role in food and fuel industry. Since aminopeptidases take part in many physiological processes in all organisms, it is important to learn their role and characteristics in economically relevant plants. Extracts of non-germinated winter rape seeds were screened for aminopeptidase activity. Substrate specificity, the influence of pH and temperature, as well as effect of protease inhibitors and chosen metal ions on the aminopeptidase activity were determined. The approximate molecular weight estimated by NATIVE-PAGE and SDS-PAGE electrophoresis was ～60 kDa. The partially purified enzyme as well as the aminopeptidases present in crude extract cleaved preferentially Phe-pNA. The activity profiles toward several substrates were also determined. Maximum activity was observed at pH 6.5 and temperature of 40 °C for Phe-pNA as a substrate. Two visible picks in the pH profile toward Phe-pNA, together with other results (IEF) suggest the presence of more than one aminopeptidase, having similar molecular mass. Much lower activity and broad pH profiles were observed for Leu- and Ala-pNA as substrates.

Thermodynamics of enzymic synthesis of solid-phase peptides

The Gibbs free energy changes of the individual and net synthetic equilibrium of solid-phase tert-butyloxycarbonyl-Phe-Gly p-substituted anilides are calculated from the HPLC analysis data for the equilibrium concentrations. A linear free energy relationship is observed for the net synthetic equilibrium and precipitation equilibrium, suggesting that the latter provides the driving force for the chymotryptic synthesis of these insoluble peptides. This conclusion is strongly supported by a linear correlation between the synthetic yield and the square root of the product solubility.