1206198-30-2Relevant academic research and scientific papers
Synthesis and crystal structure of 2'-Se-modified guanosine containing DNA
Salon, Jozef,Sheng, Jia,Gan, Jianhua,Huang, Zhen
experimental part, p. 637 - 641 (2010/04/29)
(Chemical Equation Presented) Selenium modification of nucleic acids is of great importance in X-ray crystal structure determination and functional study of nucleic acids. Herein, we describe a convenient synthesis of a new building block, the 2′-SeMe-modified guanosine (GSe) phosphoramidite, and report the first incorporation of the 2′-Se-G moiety into DNA. The X-ray crystal structure of the 2′-Se-modified octamer DNA (5′-GTGSeTACAC-3′) was determined at a resolution of 1.20 A.We also found that the 2′-Se modification points to the minor groove and that the modified and native structures are virtually identical. Furthermore, we observed that the 2′-Se-G modification can significantly facilitate the crystal growth with respect to the corresponding native DNA.
