12236-82-7

Basic information

• Product Name: REACTIVE BLUE 2
• Synonyms: BASILEN BLUE E-3G;CI NO 61211;CI 61211;CIBACRON BLUE F3GA;CIBACRON BLUE 3G-A;1-AMINO-4-[[4-[[4-CHLORO-6-[[3 (OR 4)-SULFOPHENYL]AMINO]-1,3,5-TRIAZIN-2-YL]AMINO]-3-SULFOPHENYL]AMINO]-9,10-DIHYDRO-9,10-DIOXO-2-ANTHRACENESULFONIC ACID;REACTIVE BLUE 2;PROCION BLUE HB
• CAS NO: 12236-82-7
• Molecular Formula: C29H20ClN7O11S3
• Molecular Weight: 774.16
• EINECS: 235-465-0
• Product Categories: Affinity Chromatography;Dye Resins;Protein Chromatography;C;Stains and Dyes;Stains&Dyes, A to
• Mol File: 12236-82-7.mol
• Article Data: 0

Chemical Properties

• Appearance: dark blue/saline suspension
• Density: 1.845 g/cm3
• Refractive Index: 1.777
• Storage Temp.: 2-8°C
• Solubility: H2O: soluble
• Stability: Stable. Incompatible with strong oxidizing agents.
• CAS DataBase Reference: REACTIVE BLUE 2(CAS DataBase Reference)
• NIST Chemistry Reference: REACTIVE BLUE 2(12236-82-7)
• EPA Substance Registry System: REACTIVE BLUE 2(12236-82-7)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 12236-82-7(Hazardous Substances Data)

Usage

Chemical Properties

dark blue powder

Preparation

1-Amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid and 2,5-Diaminobenzenesulfonic acid condensation, again with 2,4,6-Trichloro-1,3,5-triazine condensation, and 4-Aminobenzenesulfonic acid or 3-Aminobenzenesulfonic acid condensation.

Enzyme inhibitor

This reactive dye (FWtrisodium-salt = 840.11 g/mol; lmax = 607 nm), also known as Reactive Blue 2 and Cibacron Blue F3G-A, is a sulfonated triazine often used to probe nucleotide binding sites. Procion Blue H-B is also a P2Y purinoceptor antagonist and is one of the most potent antagonists for ATPactivated channels (See also Cibacron Blue). When immobilized on a support matrix, this triazine can be used to purify proteins that bind nucleotides or nucleotide-containing coenzymes. Note: Given its tendency to bind in place of nucleotides, this agent is apt to have inhibitory effects beyond those listed below. Target(s): aldehyde reductase; alkaline phosphatase; ATP-activated channels; carbonyl reductase; choline Oacetyltransferase; choline-phosphate cytidylyltransferase; coenzyme F420 hydrogenase; ferredoxin:NADP+ oxidoreductase; glucose-6- phosphate dehydrogenase; glutathione S-transferase; hexokinase; NAD+ nucleosidase, or NADase, or NAD+ glycohydrolase; nucleotide diphosphatase; phosphoglycerate kinase; P2Y purinoceptor; thymidylate synthase; and UDP-glucose 4- epimerase.

Properties and Applications

bright blue. Blue powder, have good solubility in water, not sensitive to that fights. In dye bath of iron ion in color shade darker; Copper ions in colored light turned green. Used for cellulose fiber, polyamide, wool, silk dyeing and printing. Standard Light Fastness Oxygen bleaching Persperation Fastness Soaping Fading Stain Fading Stain Fading Stain ISO 6-7 4-5 5 4-5 5 4 5

Standard

Light Fastness

Fading

Stain

ISO

6-7

InChI:InChI=1/C23H16N6O8S2/c24-20-17(39(35,36)37)8-15(18-19(20)22(31)13-4-2-1-3-12(13)21(18)30)28-11-5-6-14(16(7-11)38(32,33)34)29-23-26-9-25-10-27-23/h1-10,28H,24H2,(H,32,33,34)(H,35,36,37)(H,25,26,27,29)