122406-02-4 Usage
Description
2',3'-dideoxyribosylinosine 5'-triphosphate, also known as ddITP, is a nucleoside triphosphate analog that plays a crucial role in various molecular biology techniques. It is a synthetic compound characterized by the absence of the 3'-hydroxyl group in its ribose, which imparts resistance to further elongation by DNA polymerase. This distinctive feature enables ddITP to terminate DNA synthesis during sequencing processes, facilitating the determination of the nucleotide base order in a DNA molecule. Moreover, its incorporation into DNA during PCR makes it a valuable tool in mutation detection assays, underscoring its significance in molecular biology and genetic research.
Uses
Used in DNA Sequencing:
2',3'-dideoxyribosylinosine 5'-triphosphate is used as a terminator in enzymatic DNA sequencing for the precise determination of nucleotide base order. Its inability to allow further elongation by DNA polymerase upon incorporation enables the identification of the sequence of bases in a DNA molecule.
Used in Mutation Detection Assays:
In the field of genetic research, 2',3'-dideoxyribosylinosine 5'-triphosphate is utilized as an incorporation agent during PCR. Its use in this context aids in the detection of mutations by causing premature termination of DNA synthesis at the site of the mutation, thereby facilitating the identification of genetic variations.
Used in Molecular Biology Research:
2',3'-dideoxyribosylinosine 5'-triphosphate is employed as a key component in various molecular biology experiments. Its unique properties make it instrumental in studying DNA replication, DNA repair mechanisms, and the development of novel diagnostic and therapeutic strategies in genetic medicine.
Check Digit Verification of cas no
The CAS Registry Mumber 122406-02-4 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,2,4,0 and 6 respectively; the second part has 2 digits, 0 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 122406-02:
(8*1)+(7*2)+(6*2)+(5*4)+(4*0)+(3*6)+(2*0)+(1*2)=74
74 % 10 = 4
So 122406-02-4 is a valid CAS Registry Number.