122965-43-9

Basic information

• Product Name: METHYLENE BLUE
• Synonyms: CI 52015;CINO 52015;METHYLENE BLUE HYDRATE;Basic Blue 9~C.I. 52015;C.I. 52015 hydrate;METHYLENE BLUE FOR MICROSCOPY;METHYLENE BLUE REDOX INDICATOR, REAG. PH . EUR.;METHYLENE BLUE TRIHYDRATE MOLECULAR*BIOL OGY REAGENT
• CAS NO: 122965-43-9
• Molecular Formula: C16H20ClN3OS
• Molecular Weight: 337.87
• EINECS: 200-515-2
• Product Categories: Laser DyesStains and Dyes;M;Organic Electronics and Photonics;Photonic and Optical Materials;Stains&Dyes, A to;Nitric Oxide and Cell Stress;Nitric Oxide Metabolism;Nitric Oxide Scavengers;Nitric Oxide ScavengersCell Signaling Enzymes;Indicators;Redox Indicators;Titration;Analytical Reagents;Microscopy Reagents;Stains and Dyes
• Mol File: 122965-43-9.mol
• Article Data: 0

Chemical Properties

• Melting Point: 190 °C
• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: Green/Fine Crystalline Powder
• Density: g/cm³
• Storage Temp.: room temp
• Solubility: H2O: soluble4mg/4ml
• Water Solubility: Soluble in water, alcohol, acetic acid, glycerol and chloroform. Insoluble in ether.
• Merck: 14,6060
• BRN: 3599847
• CAS DataBase Reference: METHYLENE BLUE(CAS DataBase Reference)
• NIST Chemistry Reference: METHYLENE BLUE(122965-43-9)
• EPA Substance Registry System: METHYLENE BLUE(122965-43-9)

Safety Data

• Hazard Codes: Xn
• Statements: 22
• Safety Statements: 26-39-61
• RIDADR: UN 2811 6.1/PG 3
• WGK Germany: 3
• RTECS: SO5600000
• TSCA: Yes
• Hazardous Substances Data: 122965-43-9(Hazardous Substances Data)

Usage

Chemical Description

Methylene blue is a photosensitizer that can absorb light and transfer energy to other molecules.

Description

Methylene Blue (MB) is a basic histological dye, known for its green fine crystalline powder form. It is a versatile compound with a wide range of applications across various industries due to its unique properties.

Uses

Used in Staining Procedures:
Methylene Blue is used as a dye in different staining procedures such as Wright's stain and Jenner's stain. These staining techniques are essential for the examination of various biological samples under the microscope.
Used in Molecular Biology:
Methylene Blue serves as a temporary staining agent to examine RNA or DNA under the microscope or in a gel. This allows researchers to visualize and analyze the structure and integrity of these essential biomolecules.
Used in Analytical Chemistry:
As a redox indicator, Methylene Blue is widely used in analytical chemistry. It helps in determining the presence and concentration of various chemical species by undergoing color changes in response to changes in oxidation states.
Used in Organic Chemistry:
Methylene Blue acts as a photosensitizer, generating singlet oxygen through a Diels-Alder reaction. This property makes it useful in the synthesis of organic peroxides, which are important intermediates in the chemical industry.
Used in Sulfur Analysis:
In the field of analytical chemistry, Methylene Blue is also employed in sulfide analysis. It aids in the detection and quantification of sulfide ions, which are crucial in various environmental and industrial applications.
Used in Aquaculture:
Methylene Blue plays an important role in aquaculture, particularly in treating fungal infections in fish and other aquatic organisms. Its antimicrobial properties make it an effective treatment option for various fungal pathogens.

Biochem/physiol Actions

Methylene blue is used as a bacteriologic stain or dye and indicator. It mediates the inactivation of enveloped viruses in plasma, when exposed to visible light. It is an inhibitor of nitric oxide synthase and guanylate cyclase. It also plays a key role in medicine as an antimalarial agent, reducing agent in methemoglobinemia and inducer of apoptosis in cancer cells.

InChI:InChI=1/C16H18N3S.ClH.3H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;;;/h5-10H,1-4H3;1H;3*1H2/q+1;;;;/p-1